Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Barley modified for increased (1,3-1,4)-β-glucan degradation
EN
pJH271-Beta-Glu-307
No
-
Person:Justus-Liebig-Universität Gießen, Institut für Phytopathologie und Angewandte ZoologieHeinrich-Buff-Ring 26-32Gießen, Hessen
35392, GermanyPhone: + 49 641 99-37490,Fax: + 49 641 99-37499,Related OrganizationJustus-Liebig-Universität Gießen ()Heinrich-Buff-Ring 26-32Gießen, Hessen
35392, GermanyPhone: + 49 641 99-37490,Fax: + 49 641 99-37499,
Barley was modified with the insertion of a chimeric (1,3-1,4)-β-glucanase leading to increased degradation of (1,3-1,4)-β-glucane in the endosperm and aleuron during germination.
This allows α-amylases and proteases to access starch and storage proteins that are stored in the endosperm, and therefore providing carbohydrates and amino acids to the growing shoot. This increases the nutritional profile of this barley when used as feed. For usage in beer brewing, where barley grains are heated, the expressed (1,3-1,4)-β-glucanase is thermostable.
EN
This allows α-amylases and proteases to access starch and storage proteins that are stored in the endosperm, and therefore providing carbohydrates and amino acids to the growing shoot. This increases the nutritional profile of this barley when used as feed. For usage in beer brewing, where barley grains are heated, the expressed (1,3-1,4)-β-glucanase is thermostable.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-12110-5 Organism Hordeum vulgare (Barley, HORVU)Crops
EN
pJH271
EN
- Agrobacterium-mediated DNA transfer
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
-
BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-45846-5 Green Fluorescent Protein gene | Aequorea victoria (Crystal Jellyfish, Water Jellyfish, AEQVI)Protein coding sequence | Selectable marker genes and reporter genes
-
BCH-GENE-SCBD-108896-1 Hor3-1 gene promoter | Hordeum vulgare (Barley, HORVU)Promoter
-
BCH-GENE-SCBD-108897-1 Hor3-1 transit peptide | Hordeum vulgare (Barley, HORVU)Transit signal
-
BCH-GENE-SCBD-108898-1 Beta-1,3-1,4-glucanase geneProtein coding sequence | Changes in quality and/or metabolite content (Carbohydrates)
-
BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
The chimeric (1,3-1,4)-β-glucanase gene was synthesised through the intragenic recombination of two (1,3-1,4)-β-glucanases, specifically amino acids 1-12 of the glucanaseBacillus amyloliquefaciens and amino acids 13-214 of glucanase from Bacillus macerans.
The nucleotide sequence was codon-optimized to ensure functionality of the microbial gene in plants by raising the GC content to 63%. The resulting enzyme has the same substrate specificity as it homologous barley counterpart but has enhanced thermostability.
Te expression cassetes were cloned in to the pBIN19 vector and also includes LB and RB of Agrobacterium tumefaciens, parts of the lacZ gene from E. coli and fragments of the bacteriophage M13 origin of replication, which are not functional in plants, as well as fragments of the nopaline synthase gene promoter from Agrobacterium tumefaciens.
The expression of green fluorescent protein (GFP) and bialaphos resistance gene (bar) serve as selection markers
EN
The nucleotide sequence was codon-optimized to ensure functionality of the microbial gene in plants by raising the GC content to 63%. The resulting enzyme has the same substrate specificity as it homologous barley counterpart but has enhanced thermostability.
Te expression cassetes were cloned in to the pBIN19 vector and also includes LB and RB of Agrobacterium tumefaciens, parts of the lacZ gene from E. coli and fragments of the bacteriophage M13 origin of replication, which are not functional in plants, as well as fragments of the nopaline synthase gene promoter from Agrobacterium tumefaciens.
The expression of green fluorescent protein (GFP) and bialaphos resistance gene (bar) serve as selection markers
EN
- Research
EN
For transformation of pJH271-Beta-Glu-307 the barley variety Golden promise was used. By crossing with the elite line Baronesse for several generations the genetic background changed, however, a few alleles from Golden Promise are reported to be introgressed into the genome.
EN
- Transcriptome and metabolome profiling of field-grown transgenic barley lack induced differences but show cultivar-specific variances, PNAS [ English ]
- Transcriptome and metabolome profiling of field-grown transgenic barley lack induced differences but show cultivar-specific variances [ German ]
- The production of recombinant proteins in transgenic barley grains [ English ]
- The production of recombinant proteins in transgenic barley grains [ German ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
